Ƙirƙirar Samfurin Mouse na Fasaha na Gaba-gaba
Fasahar TurboMice™
MingCeler ya haɓaka ta hanyar haɓakar haɓakar tetraploid.
Ta hanyar haɗawaMadaidaicin fasahar Gyaran Halittuda ingantaccen linzamin kwamfutaFasahar Shiri Kwayoyin Embryonic Stem Cell, yanzu za mu iya gyara kusan kowane wuri mai niyya.
Fasahar TurboMice™
Fasahar Haɗin Tetraploid ɗinmu mai inganci ta ƙyale mu mu ƙara yawan haifuwar beraye daga 1% -5% zuwa 30% -60% tare da gyare-gyaren kwayoyin halitta, kusan dacewa da ingancin canjin amfrayo na yau da kullun.
MingCeler shine kamfani na farko a duniya don samun nasarar cim ma canjin fasahar Tetraploid Complementation daga dakin gwaje-gwaje zuwa aikace-aikacen masana'antu.
Fasahar Gargajiya
Fasahar ƙirar dabba ta gargajiya, kamar pronuclear microinjection da ES niyya, ya ƙunshi kiwo aƙalla tsara 2 zuwa 3 don samun berayen genotype na homozygous, wanda yawanci yana ɗaukar watanni 6-8.
Wannan tsayin tsari yana da matuƙar hana ci gaba da ingantaccen sabbin ci gaban ƙwayoyi da binciken cututtuka.
Batch mai sauri
1. har zuwa 20 berayen da aka gyara masu homozygous a cikiWatanni 2-4.
2. ≈ 50 homozygous beraye a cikin wadannanWata 2.
3. 400+ homozygous beraye a ciki8-12 watanni.
Rahoton Harka a cikin 2020
An yi amfani da fasahar TurboMice™ don samarwa cikin sauri500 homozygousberayen ACE2 na mutum a cikin kawaiwatanni 8don COVID-19 magani da nazarin rigakafi.
Zaɓin Ƙirar Maɗaukaki
Dabarun gargajiya suna da ƙarancin iyaka a cikin Maballin Maballin Mita, yayin da Fasaha ta Balbarice ta ba zaɓa daga (gami da balbor / c, icr, da sauransu).
Berayen ƙarni na F0 waɗanda aka gina ta amfani da fasahar TurboMice™ sune berayen da aka yi niyya tare da asalin tantai ɗaya.
● Saboda haka, kwayoyin halitta na mice F0 iri ɗaya ne, yana rage kurakuran gwaji da ke haifar da bambancin kayan halitta.
● Saboda haka, bayanan gwaji don kimanta ingancin magunguna da sauran gwaje-gwajen sun fi dacewa da dogaro.
Yana Kiyaye Kyawun Mutun Halitta
A cikin Situ Precision Gene Editing
● A wurin daidaitaccen gyaran kwayoyin halitta tare da madaidaicin matakan bayyana kwayoyin halitta da takamaiman takamaiman nama.
● Berayen ACE2 na ɗan adam waɗanda aka haɓaka ta hanyar dabarun gargajiya ana haifar da su ta hanyar gabatarwar waje na mai tallata K18-ACE2, wanda ba zai iya cimma daidaitaccen gyara ba saboda shigar da bazuwar, wanda ya haifar da ƙarancin takamaiman nama na ACE2 magana a cikin ƙirar linzamin kwamfuta na ACE2. .
● Berayen ACE2 na MingCeler na ɗan adam suna nuna takamaiman magana a cikin gabobin daban-daban (hotunan C da D a sama), kuma yadda ya kamata ke daidaita halayen asibiti bayan kamuwa da cutar SARS-CoV-2.
Platform na Musamman EnhancerPlus
●Dandalin EnhancerPlus na MingCeler na iya taimaka wa abokan cinikinmu su inganta matakan maganganun kwayoyin halitta.
● Sakamakon shekaru na tara bayanai a cikin epigenetics da binciken ƙaddarar ƙaddarar kwayar halitta, dandalin MingCeler's EnhancerPlus zai iya yin hasashen daidai matsayin matsayin masu haɓakawa, yin ƙira na dabarun gyara kwayoyin halitta bisa tushen shaida.
A sakamakon haka, kwayoyin halittar da aka yi niyya za su iya kama da yanayin magana da matakan kwayoyin halitta.
Rahoton shari'a: Humanization na X gene.
● Yin amfani da dandamali na EnhancerPlus, dabarun da aka inganta da kuma matakin magana ya ga karuwar umarni uku na girma (duba hoto na sama), ya kai ga buƙatun ci gaban ƙwayoyi kamar yadda abokin ciniki ya ƙaddara a matakin furotin.
● Bayan kammala taro na farko na duniya na ACE2 ɗan adam a cikin 2020, MingCeler ya haɓaka ƙirar linzamin kwamfuta ta ACE2 ta hanyar haɓakawa huɗu ta hanyar haɓakawa na EnhancerPlus, tare da matakin magana na ɗan adam ACE2 ƙarshe ya kai kusa da ƙarshen magana na linzamin kwamfuta ACE2.
Matakan bayyanawa na ɗan adam ACE2 a cikin gabobin daban-daban na ƙirar linzamin kwamfuta na ɗan adam
Multi-Locus Gene Editing
● Fasahar TurboMice ™ ita ce mafi kyawun zaɓi a kasuwa don samar da nau'ikan linzamin kwamfuta da aka gyara na wurare da yawa, yana ba da fa'idodin babban daidaito wajen shigar da wuri, dogon saka guntu, da gyare-gyaren ƙwayoyin halitta da yawa, ba tare da matsalolin rarrabuwa ba.
Fasahar TurboMice ™ tana ba da damar yin gyare-gyaren lokaci guda har zuwa kwayoyin halitta guda 3 don samar da samfuran homozygous Multi-locus gene-edited linzamin kwamfuta kai tsaye daga sel da aka gyara na mahaifa, ba tare da buƙatar tsarin kiwo / tantancewa ba, yana ba da nau'ikan iri-iri. na manyan ƙima masu ƙima da ake buƙata don ingantaccen bincike na magani.
MingCeler ya sami nasarar haɓaka nau'ikan linzamin kwamfuta daban-daban da aka gyara a cikin ɗan gajeren lokaci, dangane da ACE2 na mice.
Dogon Rubuce-rubucen Halitta
Fasahar TurboMice ™ tana ba da damar daidaitaccen gyaran kwayoyin halitta na dogon gutsuttsura sama da 20kb, yana sauƙaƙa saurin samar da hadaddun samfura kamar ɗan adam, yanayin ƙwanƙwasa (CKO), da babban guntun ƙwanƙwasa (KI).
Labarai
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin yana ɗaure ACE2 don toshe hulɗar sa tare da furotin mai karu na SARS-CoV-2 kuma yana da tasiri wajen hana kamuwa da cutar SARS-CoV-2 a cikin samfuran dabbobi.Cell Res.2021 Janairu; 31 (1): 17-24.doi: 10.1038/s41422-020-00450-0.(IN: 20.507)
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT.Natl Sci Rev. 2020 Nov 24;8(2): nwaa285.doi: 10.1093/nsr/nwa285.(IN: 16.693)
Gudun Sabis
Duk abin da kuke buƙatar ku yi shine samar mana da buƙatun ku na gyaran kwayoyin halitta don ƙirar linzamin kwamfutanku.Dangane da ƙayyadaddun ku, za mu haɗa tsarin farko na musamman, kuma bayan ƙarin tattaunawa da yarjejeniyar juna, za mu sanya hannu kan kwangilar sabis na fasaha.Bayan fara aikin za mu samar da sabuntawa akan lokaci akan ci gaba.